OLIG2 antibody (AA 1-141)

Catalog No. ABIN7658224

This Mouse Monoclonal antibody specifically detects OLIG2 in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for OLIG2 antibody (AA 1-141) (ABIN7658224)

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This OLIG2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: OLIG2-2400

Product Details anti-OLIG2 Antibody

Binding Specificity: AA 1-141

Purpose: OLIG2 (Marker of Glial Brain Tumors) (OLIG2/2400)

Characteristics: Olig2, a basic helix-loop-helix transcription factor, is involved in oligodendroglial specification. Olig2 expression has been reported in most glial tumors, such as oligodendrogliomas and astrocytomas. Although more than half of glioblastomas are positive for Olig2, expression is very weak in terms of both percentage of labeled cells and intensity. No Olig2 expression has been found in the non-glial tumors including neuro-epithelial tumors, ependymomas, sub-ependymomas, medulloblastomas, and non-neuroepithelial tumors, such as CNS lymphomas, meningiomas, schwannomas, atypical teratoid / rhabdoid tumor, and haemangioblastomas. Compared to the strong staining seen in glioma samples, a weak expression is observed in non-tumoral brain tissue (gliosis).Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human OLIG2 protein (around aa 1-141)

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: THP-1 cells. Astrocytoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for OLIG2

Target: OLIG2 (Oligodendrocyte Lineage Transcription Factor 2 (OLIG2))

Alternative Name: OLIG2

Background:

Synonyms: Basic helix loop helix protein class B1 (bHLHB1), basic helix-loop-helix protein 19 (bHLHe19), OLIG2, Oligodendrocyte lineage transcription factor 2, Oligodendrocyte specific bHLH transcription factor 2, Oligodendrocyte transcription factor 2, Protein kinase C-binding protein 2 (PRKCBP2), RACK17

Gene Symbol: OLIG2

Tissue Expression: Glia

Molecular Weight: 30-40 kDa

Gene ID: 10215

UniProt: Q13516