P-Cadherin antibody (CF®740)

Catalog No. ABIN7658271

This anti-P-Cadherin antibody is a Mouse Monoclonal antibody detecting P-Cadherin in WB, IHC, IF, IP, Func and IHC (fp). Suitable for Human.

Quick Overview for P-Cadherin antibody (CF®740) (ABIN7658271)

Target: P-Cadherin (CDH3)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This P-Cadherin antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: 6A9

Product Details anti-P-Cadherin Antibody (CF®740)

Purpose: P-Cadherin (CDH3) (6A9), CF740 conjugate

Characteristics: This antibody recognizes a protein of 116 kDa, identified as P-Cadherin-1 (CDH3). It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congenital hypotrichosis with juvenile macular dystrophy.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A431 trypsinized membranes

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: A431 or PC-3 cells. Pancreas, Placenta or Prostate

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for P-Cadherin

Target: P-Cadherin (CDH3)

Alternative Name: P-Cadherin

Background:

Synonyms: Cadherin 3 type 1, Cadherin-3, Cadp, Calcium dependent adhesion protein placental, CDH3, CDHP, HJMD, P-cadherin (Placental), PCAD, Placental cadherin

Gene Symbol: CDH3

Tissue Expression: Epithelial cells

Molecular Weight: 116 kDa

Gene ID: 1001

UniProt: P22223