Recombinant PAX5 antibody (CF®405S)

Catalog No. ABIN7658286

This anti-PAX5 antibody is a Mouse Monoclonal antibody detecting PAX5 in WB. Suitable for Human.

Quick Overview for Recombinant PAX5 antibody (CF®405S) (ABIN7658286)

Target: PAX5 (Paired Box 5 (PAX5))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX5 antibody is conjugated to CF®405S

Application: Western Blotting (WB)

Clone: RPAX5-2060

Product Details anti-PAX5 Antibody (CF®405S)

Purpose: PAX5 / BSAP (Early B-Cell Marker)(rPAX5/2060), CF405S conjugate

Characteristics: The specificity of this monoclonal antibody to its intended target was validated by HuProt™, Array, containing more than 19, 000, full-length human proteins. The PAX5 gene is a member of thepaired box (PAX)family of transcription factors. The central feature of this gene family is a novel, highly conservedDNA-binding domain, known as thepaired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute toneoplastic transformation. The PAX5 gene encodes theB-celllineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development andspermatogenesis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length humanPAX5 protein

Isotype: IgG1, kappa

Application Details

Application Notes: ELISA: For coating, purchase antibody without BSA, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: Daudi or Ramos cells. Tonsil, spleen, Lymph Nodeor Colon Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for PAX5

Target: PAX5 (Paired Box 5 (PAX5))

Alternative Name: BSAP

Background:

Synonyms: B cell lineage specific activator, BSAP, EBB-1, KLP, Paired box 5

Gene Symbol: PAX5

Tissue Expression: B-cells

Molecular Weight: 42 kDa

Gene ID: 5079

UniProt: Q02548