PAX2A antibody (AA 223-354) (CF®740)

Catalog No. ABIN7658383

This Mouse Monoclonal antibody specifically detects PAX2A in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for PAX2A antibody (AA 223-354) (CF®740) (ABIN7658383)

Target: PAX2A (Paired Box Gene 2a (PAX2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX2A antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PAX2-1104

Product Details anti-PAX2A Antibody (CF®740)

Binding Specificity: AA 223-354

Purpose: PAX2 (Renal Cell & Ovarian Carcinoma Marker) (PAX2/1104), CF740 conjugate

Characteristics: This antibody recognizes a protein of 42 kDa, which is identified as PAX2. It is a member of the paired box family of transcription factors, which is required for development and proliferation of the kidney, brain, and MA1/4llerian organs. PAX2 genes contain a highly conserved DNA sequence within the paired box region, which encodes a DNA-binding domain, enabling PAX proteins to bind the promoters of specific genes to transcriptionally regulate their expression. PAX2 is specifically expressed in the developing central nervous system, eye, ear, and urogenital tract, and is essential for the development of these organs. In normal adult tissues PAX2 was mainly detected in the urogenital system, including kidney, ureteric epithelium, fallopian tube epithelium, ovary and uterus. In tumors, PAX2 has been detected in renal cell carcinomas, Wilms' tumors, nephrogenic adenomas and papillary serous carcinoma of the ovary. PAX2 has been used as a marker for the identification of renal cell carcinoma and ovarian carcinoma by immunohistochemistry. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 223-354) human PAX2 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: NAMALWA cells. Fetal kidney or Renal Cell Carcinoma (RCC) or Ovarian Carcinoma.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for PAX2A

Target: PAX2A (Paired Box Gene 2a (PAX2A))

Alternative Name: PAX2

Background:

Synonyms: Paired box gene 2, paired box homeotic gene 2, paired box protein 2, Paired box protein Pax-2, Paired box protein Pax2

Gene Symbol: PAX2

Tissue Expression: Uterus|Kidney|Ovary

Molecular Weight: 42 kDa

Gene ID: 5076

UniProt: Q02962

Pathways: Carbohydrate Homeostasis, Stem Cell Maintenance, Tube Formation