Perforin 1 antibody (AA 413-552)

Catalog No. ABIN7658547

This anti-Perforin 1 antibody is a Mouse Monoclonal antibody detecting Perforin 1 in IHC and IHC (fp). Suitable for Human.

Quick Overview for Perforin 1 antibody (AA 413-552) (ABIN7658547)

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Perforin 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PRF1-2470

Product Details anti-Perforin 1 Antibody

Binding Specificity: AA 413-552

Purpose: Perforin-1 (Pore Forming Protein) (Apoptosis Marker)(PRF1/2470)

Characteristics: Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8 T-cells, natural killer cells and some CD4 T-cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Perforin-1 protein fragment (around aa 413-552)

Isotype: IgG2c, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HDLM-2 cells. Human spleen tissue (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Perforin 1

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin-1

Background:

Synonyms: Cytolysin, FLH2, HPLH2, Lymphocyte pore-forming protein, PRF1 (pore forming protein 1), Perforin-1, PFP, PGFL, PIGF, PIGF-2, PLGF

Gene Symbol: PRF1

Tissue Expression: NK cells|T-cells

Molecular Weight: 75 kDa

Gene ID: 5551

UniProt: P14222

Pathways: Apoptosis, Caspase Cascade in Apoptosis