Peroxiredoxin 4 antibody (CF®405S)

Catalog No. ABIN7658575

This Mouse Monoclonal antibody specifically detects Peroxiredoxin 4 in . It exhibits reactivity toward Human.

Quick Overview for Peroxiredoxin 4 antibody (CF®405S) (ABIN7658575)

Target: Peroxiredoxin 4 (PRDX4)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Peroxiredoxin 4 antibody is conjugated to CF®405S

Application: Please inquire

Clone: CPTC-PRDX4-2

Product Details anti-Peroxiredoxin 4 Antibody (CF®405S)

Purpose: Peroxiredoxin 4 (Prognostic Marker for Lung SqCC)(CPTC-PRDX4-2), CF405S conjugate

Characteristics: The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX IV gene is expressed in many tissues. It exists as a precursor protein, which is only detected in testis, and aprocessed secreted form. PRX IV is highly expressed in lung cancer and is necessary for the promotion of lung cancer in vitro. Studies have demonstrated that PRX IV positive expression is significantly correlated with recurrences and shorter disease-free survival in patients with early-stage lung squamous cell carcinoma, and therefore can be used as a prognostic marker in lung squamous cell carcinoma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human PRDX4 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: HepG2, HeLa and Jurkat cell lysates. Ovarian carcinoma tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Peroxiredoxin 4

Target: Peroxiredoxin 4 (PRDX4)

Alternative Name: Peroxiredoxin 4

Background:

Synonyms: Antioxidant enzyme AOE372, EC 1.11.1.15, Thioredoxin dependent peroxide reductase A0372, TRANK

Gene Symbol: PRDX4

Molecular Weight: 27 kDa

Gene ID: 10549

UniProt: Q13162