Plakophilin 1 antibody (N-Term) (CF®740)

Catalog No. ABIN7658623

This Mouse Monoclonal antibody specifically detects Plakophilin 1 in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for Plakophilin 1 antibody (N-Term) (CF®740) (ABIN7658623)

Target: Plakophilin 1 (PKP1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Plakophilin 1 antibody is conjugated to CF®740

Application: Western Blotting (WB)

Clone: 10B2

Product Details anti-Plakophilin 1 Antibody (CF®740)

Binding Specificity: N-Term

Purpose: Plakophilin-1 (PKP1) (10B2), CF740 conjugate

Characteristics: This antibody recognizes a protein of 75 kDa, identified as Plakophilin-1. Its epitope maps between aa 1-27 human Plakophilin-1. Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: N-terminus of human Plakophilin-1 protein

Isotype: IgG1

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HaCat Cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Plakophilin 1

Target: Plakophilin 1 (PKP1)

Alternative Name: Plakophilin-1

Background:

Synonyms: B6P, B, 6 protein, PKP1, Plakophilin-1 (ectodermal dysplasia/skin fragility syndrome)

Gene Symbol: PKP1

Tissue Expression: Cardiac

Molecular Weight: 75 kDa

Gene ID: 5317

UniProt: Q13835