Plakophilin 1 antibody (N-Term)

Catalog No. ABIN6938188

The Mouse Monoclonal anti-Plakophilin 1 antibody (Clone 10B2) (ABIN6938188) specifically detects Plakophilin 1 in WB, IF and FACS. The antibody is reactive with Human and Mouse samples.

Quick Overview for Plakophilin 1 antibody (N-Term) (ABIN6938188)

Target: Plakophilin 1 (PKP1)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Plakophilin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 10B2

Product Details anti-Plakophilin 1 Antibody

Binding Specificity: N-Term

Purpose: Plakophilin-1 (PKP1) Antibody

Specificity: Recognizes a protein of 75 kDa, identified as Plakophilin-1. Its epitope maps between aa 1-27 human Plakophilin-1. Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.

Cross-Reactivity (Details): Human. Mouse.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: N-terminus of human Plakophilin-1 protein

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), Optimal dilution for a specific application should be determined.

Positive_Control: U87, HeLa and A431 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Plakophilin 1

Target: Plakophilin 1 (PKP1)

Alternative Name: PKP1

Background:

B6P, Band 6 protein, PKP1, Plakophilin-1 (ectodermal dysplasia/skin fragility syndrome)

HeLa and A431 cells., U87

Molecular Weight: 75kDa

Gene ID: 5317, 497350

UniProt: Q13835