PAK1/2/3 antibody (pThr423)

Catalog No. ABIN732548

The Rabbit Polyclonal anti-PAK1/2/3 antibody has been validated for ELISA, WB, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect PAK1/2/3 in samples from Human.

Quick Overview for PAK1/2/3 antibody (pThr423) (ABIN732548)

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1/2/3 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PAK1/2/3 Antibody

Binding Specificity: pThr423

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of (Thr423)

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PAK1/2/3

Target: PAK1/2/3 (P21-Activated Kinases 1/2/3 (PAK1/2/3))

Alternative Name: PAK1/2/3

Background:

Synonyms: PAK1 + PAK2 + PAK3 phospho T423, Alpha PAK, Beta PAK, Gamma PAK, Oligophrenin 3, OPHN3, p21 activated kinase 1, p21 Activated Kinase 1 + 2 + 3, p21 activated kinase 2 , p21 activated kinase 3, P65 PAK, PAK1, PAK2, PAK3, PAK3beta, PAK65, PAKalpha, PAKgamma, S6/H4 kinase, Serine/threonine protein kinase PAK 1, Serine/threonine protein kinase PAK 2, Serine/threonine protein kinase PAK 3, Serine/threonine protein kinase PAK1, Serine/threonine protein kinase PAK2, Serine/threonine protein kinase PAK3, PAK1_HUMAN, PAK2_HUMAN, PAK3_HUMAN.

Background: The p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell.P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of serine 141 (serine 144 for PAK 1 and serine 139 PAK 3), catalyzed by Cdc42, is required for activation of PAK.

Gene ID: 5058