Prohibitin antibody (AA 167-261) (CF®405S)

Catalog No. ABIN7659014

The Mouse Monoclonal anti-Prohibitin antibody has been validated for WB, IHC and IHC (fp). It is suitable to detect Prohibitin in samples from Human.

Quick Overview for Prohibitin antibody (AA 167-261) (CF®405S) (ABIN7659014)

Target: Prohibitin (PHB)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prohibitin antibody is conjugated to CF®405S

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: PHB-3227

Product Details anti-Prohibitin Antibody (CF®405S)

Binding Specificity: AA 167-261

Purpose: Prohibitin (Mitochondrial Marker)(PHB/3227), CF405S conjugate

Characteristics: Recognizes a protein of 30 kDa which is identified as Prohibitin, an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although prohibitin mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. Prohibitin is an ideal mitochondrial marker. It shows antiproliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human PHB protein fragment (aa167-261)

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Liver, Kidney, Heart or Endometrium (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Prohibitin

Target: Prohibitin (PHB)

Alternative Name: Prohibitin

Background:

Synonyms: Epididymis luminal protein 215, Epididymis secretory sperm binding protein Li 54e, HEL 215, HEL S 54e, PHB, PHB1, Prohibitin

Gene Symbol: PHB

Molecular Weight: ~30 kDa

Gene ID: 5245

UniProt: P35232

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling