Prolactin Receptor antibody (CF®740)

Catalog No. ABIN7659176

The Mouse Monoclonal anti-Prolactin Receptor antibody has been validated for IHC and IHC (fp). It is suitable to detect Prolactin Receptor in samples from Human.

Quick Overview for Prolactin Receptor antibody (CF®740) (ABIN7659176)

Target: Prolactin Receptor (PRLR)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prolactin Receptor antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: B6-2

Product Details anti-Prolactin Receptor Antibody (CF®740)

Purpose: Prolactin Receptor(B6.2), CF740 conjugate

Characteristics: This antibody recognizes a protein of 70 kDa, identified as prolactin receptor. Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor), which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Semi-purified human prolactin receptor

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Testicular Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Prolactin Receptor

Target: Prolactin Receptor (PRLR)

Alternative Name: Prolactin Receptor

Background:

Synonyms: CPRLP|hPRL receptor|hPRLrI|Lactogen receptor|OPR|Pr-1|Pr-3|PRL-R|Prolactin receptor delta 7/11|RATPRLR|Secreted prolactin binding protein|Truncated testis-specific box 1-C prolactin receptor

Gene Symbol: PRLR

Molecular Weight: 70 kDa

Gene ID: 5618

UniProt: P16471

Pathways: JAK-STAT Signaling, Response to Growth Hormone Stimulus