SPTAN1 antibody (AA 2351-2475) (CF®640R)

Catalog No. ABIN7660133

The Mouse Monoclonal anti-SPTAN1 antibody has been validated for IHC and IHC (fp). It is suitable to detect SPTAN1 in samples from Human.

Quick Overview for SPTAN1 antibody (AA 2351-2475) (CF®640R) (ABIN7660133)

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPTAN1 antibody is conjugated to CF®640R

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: SPTAN1-3351

Product Details anti-SPTAN1 Antibody (CF®640R)

Binding Specificity: AA 2351-2475

Purpose: Fodrin / Alpha Spectrin II (SPTAN1) / NEAS(SPTAN1/3351), CF640R conjugate

Characteristics: Spectrin, an actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of nonhomologous and chains, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers, and higher polymers. Spectrin I and spectrin I are present in erythrocytes, whereas spectrin II (also designated fodrin ) and spectrin II (also designated fodrin ) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Activation of calpain results in the breakdown of spectrin II, a neuronal cytoskeleton protein. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human SPTAN1 protein (around aa 2351-2475)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: Human colon or kidney tissues (IHC).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for SPTAN1

Target: SPTAN1 (Spectrin alpha Chain, Brain (SPTAN1))

Alternative Name: Alpha Spectrin II

Background:

Synonyms: Alpha-II spectrin, brain, EIEE5, Fodrin alpha chain, NEAS, Non erythrocytic spectrin alpha, non-erythroid alpha chain, SPECA, Spectrin alpha chain brain, Spectrin, alpha, non-erythrocytic 1 (alpha-fodrin), Spna2, SPTA2

Gene Symbol: SPTAN1

Molecular Weight: 240 kDa

Gene ID: 6709

UniProt: Q13813

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization