SREBF2 antibody (CF®568)

Catalog No. ABIN7660437

This anti-SREBF2 antibody is a Mouse Monoclonal antibody detecting SREBF2 in . Suitable for Human.

Quick Overview for SREBF2 antibody (CF®568) (ABIN7660437)

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SREBF2 antibody is conjugated to CF®568

Application: Please inquire

Clone: SREBP2-1579

Product Details anti-SREBF2 Antibody (CF®568)

Purpose: SREBP2(SREBP2/1579), CF568 conjugate

Characteristics: The low density lipoprotein (LDL) receptor mediates the endocytic uptake of cholesterol-carrying lipoproteins, thereby controlling cholesterol levels in cells and plasma. Transcription of the LDL receptor gene is controlled by a ten base pair sequence in the 5' flanking region, designated sterol regulatory element 1 (SRE-1). When cellular sterol stores are depleted, the element is activated, the gene is transcribed and the cellular uptake of LDL increases. A set of SREbinding proteins (SREBPs) have been identified, including two basic helixloop-helix-leucine zipper (bHLH-zip) transcription factors, designated SREBP-1 and SREBP-2. SREBP-1 and SREBP-2 have been shown to have the same specificity for SRE-1 in vitro and to activate the transcription of reporter genes containing SRE-1 in the same way. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human SREBF2 protein

Isotype: IgG1, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: Ubiqutious expression in adult and fetal tissues. Hep2 or HeLa cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for SREBF2

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Alternative Name: SREBP2

Background:

Synonyms: AI608257, bHLHd2, Class D basic helix-loop-helix protein 2, OTTHUMP00000028740, Processed sterol regulatory element-binding protein 2, SRBP2_HUMAN, SREBF 2, Srebf2, SREBF2 protein, SREBP 2, SREBP2, SREBP2gc, sterol regulatory element binding factor 2, Sterol regulatory element binding transcription factor 2

Gene Symbol: SREBF2

Molecular Weight: 68/125 kDa

Gene ID: 6721

UniProt: Q12772

Pathways: Regulation of Lipid Metabolism by PPARalpha