TAL1 antibody (CF®405S)

Catalog No. ABIN7660525

This Mouse Monoclonal antibody specifically detects TAL1 in IHC, FACS, ChIP, IHC (fp) and EMSA. It exhibits reactivity toward Human.

Quick Overview for TAL1 antibody (CF®405S) (ABIN7660525)

Target: TAL1 (T-Cell Acute Lymphocytic Leukemia 1 (TAL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TAL1 antibody is conjugated to CF®405S

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), Chromatin Immunoprecipitation (ChIP), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: BTL73

Product Details anti-TAL1 Antibody (CF®405S)

Purpose: Tal1(BTL73), CF405S conjugate

Characteristics: Activation of TAL1 characterizes up to 60 % of cases of human T cell acute lymphoblastic leukemia, making it the most frequent gain-of-function mutation observed in this disorder. TAL1 (also designated SCL) is a serine phosphoprotein and basic helix-loop-helix transcription factor known to regulate embryonic hematopoiesis. This transcription factor binds as a heterodimer with E2A and HEB/HTF4 to a nucleotide sequence motif termed the E-box. In addition, leukemogenesis is accelerated dramatically by transgenic co-expression of TAL1 and the catalytic subunit of casein kinase IIHLH transcription factors. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length TAL1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: ELISA: For coating, order antibody without BSA, Western blot 0.5-1.0 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: A549 xenograft.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TAL1

Target: TAL1 (T-Cell Acute Lymphocytic Leukemia 1 (TAL1))

Alternative Name: Tal1

Background:

Synonyms: bHLHa17, Class A basic helix-loop-helix protein 17, OTTHUMP00000009563, OTTHUMP00000009564, SCL, STEM CELL LEUKEMIA HEMATOPOIETIC TRANSCRIPTION FACTOR, Stem cell protein, T cell acute lymphocytic leukemia 1, T cell acute lymphocytic leukemia 1 protein, T cell acute lymphocytic leukemia 1 protein, T cell leukemia/lymphoma 5 protein, T-cell acute lymphocytic leukemia protein 1, T-cell leukemia/lymphoma protein 5, Tal 1 product, TAL 1 protein, TAL bHLH transcription factor 1 erythroid differentiation factor, TAL-1

Gene Symbol: TAL1

Molecular Weight: 42 kDa

Gene ID: 6886

UniProt: P17542

Pathways: Stem Cell Maintenance