Thrombomodulin antibody (AA 69-194) (CF®740)

Catalog No. ABIN7660701

This anti-Thrombomodulin antibody is a Mouse Monoclonal antibody detecting Thrombomodulin in WB, IHC and IHC (fp). Suitable for Human.

Quick Overview for Thrombomodulin antibody (AA 69-194) (CF®740) (ABIN7660701)

Target: Thrombomodulin (THBD)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombomodulin antibody is conjugated to CF®740

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: THBD-1591

Product Details anti-Thrombomodulin Antibody (CF®740)

Binding Specificity: AA 69-194

Purpose: Thrombomodulin / CD141 (Endothelial Cell Marker) (THBD/1591), CF740 conjugate

Characteristics: This antibody recognizes a protein of 75 kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi's sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Thrombomodulin protein fragment (aa69-194)

Isotype: IgG2b, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. ELISA: 2-4 μg/mL for coating order Ab without BSA. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin) 1-2 μg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with1 mM EDTA. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: THP1 Cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Thrombomodulin

Target: Thrombomodulin (THBD)

Alternative Name: CD141

Background:

Synonyms: AHUS6, BDCA3, CD141, Fetomodulin, Thbd, THPH12, THRM, Thrombomodulin (TM)

Gene Symbol: THBD

Tissue Expression: Endothelial cells

Molecular Weight: 100 kDa

Gene ID: 7056

UniProt: P07204