Recombinant Thrombomodulin antibody (AA 69-194) (Biotin)

Catalog No. ABIN7660706

The Mouse Monoclonal anti-Thrombomodulin antibody has been validated for IHC and IHC (fp). It is suitable to detect Thrombomodulin in samples from Human.

Quick Overview for Recombinant Thrombomodulin antibody (AA 69-194) (Biotin) (ABIN7660706)

Target: Thrombomodulin (THBD)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Thrombomodulin antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RTHBD-1591

Product Details anti-Thrombomodulin Antibody (Biotin)

Binding Specificity: AA 69-194

Purpose: Thrombomodulin / CD141 (Endothelial Cell Marker) (rTHBD/1591), Biotin conjugate

Characteristics: This antibody recognizes a protein of 75 kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi's sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 69-194) of human Thrombomodulin (CD141) protein

Isotype: IgG1

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. ELISA: 2-4 μg/m for coating order Ab without BSA. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA pH 9.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: THP1 Cells. Placenta, Angiosarcoma, Bladder or Cervical Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Thrombomodulin

Target: Thrombomodulin (THBD)

Alternative Name: CD141

Background:

Synonyms: AHUS6, BDCA3, CD141, Fetomodulin, Thbd, THPH12, THRM, Thrombomodulin (TM)

Gene Symbol: THBD

Tissue Expression: Endothelial cells

Molecular Weight: 100 kDa

Gene ID: 7056

UniProt: P07204