Recombinant TYMS antibody (AA 60-174) (Biotin)

Catalog No. ABIN7660738

This Mouse Monoclonal antibody specifically detects TYMS in IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant TYMS antibody (AA 60-174) (Biotin) (ABIN7660738)

Target: TYMS (Thymidylate Synthetase (TYMS))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TYMS antibody is conjugated to Biotin

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: RTYMS-1884

Product Details anti-TYMS Antibody (Biotin)

Binding Specificity: AA 60-174

Purpose: Thymidylate Synthase (5-FU Resistance Marker)(rTYMS/1884), Biotin conjugate

Characteristics: Recognizes a protein of 36 kDa, identified as Thymidylate Synthase (TS) (EC 2. 1. 1. 45). It converts deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP), which is essential for DNA biosynthesis. TS is also a critical target for the fluoropyrimidines, an important group of antineoplastic drugs that are widely used in the treatment of solid tumors. Both 5-FU and fluorodeoxyuridine are converted in tumor cells to FdUMP which inactivates TS by formation of a ternary covalent complex in the presence of the folate cofactor 5, 10-methylenetetrahydrofolate. Expression of TS protein has been reported to associate with response to 5-fluorouracil (5-FU) in human colorectal, gastric, head and neck, and breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human thymidylate synthase protein fragment (around aa 60-174)

Isotype: IgG1, kappa

Application Details

Application Notes: ELISA: For coating, order antibody without BSA, Flow cytometry: 0.5-1 μg/million cells, Immunofluorescence: 0.5-1 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: MOLT4, Ramos, 5-FU-resistant colon carcinoma cell lines (NCI H630R10, NCI H630R1), 5-FU-resistant breast cancer cell lines, MCF-Ad5 and MCF-Ad10. Testicular carcinomas.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TYMS

Target: TYMS (Thymidylate Synthetase (TYMS))

Alternative Name: Thymidylate Synthase

Target Type: Viral Protein

Background:

Synonyms: dTMP synthase, TMS, TS, TSase, TYMS protein, Tyms thymidylate synthetase

Gene Symbol: TYMS

Molecular Weight: 36 kDa

Gene ID: 7298

UniProt: P04818

Pathways: Mitotic G1-G1/S Phases