TIMP3 antibody (AA 175-211) (CF®740)

Catalog No. ABIN7660863

This anti-TIMP3 antibody is a Rabbit Polyclonal antibody detecting TIMP3 in IHC and IHC (fp). Suitable for Human, Mouse, Rat, Cow, Dog and Horse.

Quick Overview for TIMP3 antibody (AA 175-211) (CF®740) (ABIN7660863)

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIMP3 antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Product Details anti-TIMP3 Antibody (CF®740)

Binding Specificity: AA 175-211

Purpose: TIMP3(Rabbit PAb), CF740 conjugate

Characteristics: TIMP3 (tissue inhibitor of metalloproteinases 3), along with family members TIMP1, TIMP2, and TIMP4, are inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix (ECM). An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. TIMP s inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-3 may be involved in regulating trophoblastic invasion of the uterus as well as in regulating remodeling of the extracellular matrix during the folding of epithelia, and in the formation, branching and expansion of epithelial tubes.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: The amino acid sequence (aa175-211), used as the immunogen for TIMP3 Ab, is 100% homologous in human, cow, dog and horse, and 94% homologous in mouse and rat.

Isotype: IgG

Application Details

Application Notes: Immunohistology formalin-fixed 1-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Placenta or breast carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TIMP3

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Alternative Name: TIMP3

Background:

Synonyms: HSMRK222, K222TA2, Protein MIG-5, SFD, Sorsby fundus dystrophy pseudo-inflammatory, TIMP metallopeptidase inhibitor 3, Tissue Inhibitor of Metalloproteinase 3

Gene Symbol: TIMP3

Molecular Weight: ~30 kDa

Gene ID: 7078

UniProt: P35625