TLR4 antibody (CF®740)

Catalog No. ABIN7660907

The Mouse Monoclonal anti-TLR4 antibody has been validated for . It is suitable to detect TLR4 in samples from Human, Rat, Dog, Pig, Guinea Pig and Monkey.

Quick Overview for TLR4 antibody (CF®740) (ABIN7660907)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human, Rat, Dog, Pig, Guinea Pig, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR4 antibody is conjugated to CF®740

Application: Please inquire

Clone: TLR4-230

Product Details anti-TLR4 Antibody (CF®740)

Purpose: CD284 / TLR4(TLR4/230), CF740 conjugate

Characteristics: This MAb reacts with human Toll-like receptor 2 (TLR4). It is a member of the Toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human TLR4 protein

Isotype: IgG2a, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: HeLa, PC12, human peripheral blood cells. Dendritic cells, macrophage and monocytes in tonsil, placenta and adrenal.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: CD284

Background:

Synonyms: ARMD10, CD284, hToll, TLR4, TOLL, Toll Endotoxin Hyporesponsiveness, Toll-like receptor 4

Gene Symbol: TLR4

Molecular Weight: 95-120 kDa

Gene ID: 7099

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins