TCF4 antibody (AA 365-671) (CF®405S)

Catalog No. ABIN7660954

This Mouse Monoclonal antibody specifically detects TCF4 in WB. It exhibits reactivity toward Human.

Quick Overview for TCF4 antibody (AA 365-671) (CF®405S) (ABIN7660954)

Target: TCF4 (Transcription Factor 4 (TCF4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TCF4 antibody is conjugated to CF®405S

Application: Western Blotting (WB)

Clone: TCF4-1705

Product Details anti-TCF4 Antibody (CF®405S)

Binding Specificity: AA 365-671

Purpose: TCF4 (Transcription Factor 4) (TCF4/1705), CF405S conjugate

Characteristics: This antibody recognizes a protein of 71 kDa, identified as Transcription Factor 4 (TCF4). It is a basic helix-loop-helix transcription factor. The protein recognizes an Ephrussi-box (E-box) binding site (CANNTG) - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed, and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human TCF4 protein fragment (around aa 365-671)

Isotype: IgG2a, kappa

Application Details

Application Notes: ELISA: Use Ab at 2-4 μg/mL for coating, Order Ab without BSA, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Comment:

Positive Control: HeLa or HepG2 cells. Bladder

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TCF4

Target: TCF4 (Transcription Factor 4 (TCF4))

Alternative Name: TCF4

Background:

Synonyms: bHLHb19, Class B basic helix-loop-helix protein 19, Immunoglobulin transcription factor 2, ITF-2, PTHS, SEF2, SL3-3 enhancer factor 2, TCF4, Transcription factor 4

Gene Symbol: TCF4

Molecular Weight: 71 kDa

Gene ID: 6925

UniProt: P15884

Pathways: WNT Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Regulation of Hormone Metabolic Process, Carbohydrate Homeostasis, Stem Cell Maintenance, Protein targeting to Nucleus