TGFA antibody (C-Term) (CF®740)

Catalog No. ABIN7661056

This Mouse Monoclonal antibody specifically detects TGFA in IHC and IHC (fp). It exhibits reactivity toward Human, Rat, Mouse and Cow.

Quick Overview for TGFA antibody (C-Term) (CF®740) (ABIN7661056)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reactivity: Human, Rat, Mouse, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This TGFA antibody is conjugated to CF®740

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: MF9

Product Details anti-TGFA Antibody (CF®740)

Binding Specificity: C-Term

Purpose: TGFalpha(MF9), CF740 conjugate

Characteristics: This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with Ab-1 is completely blocked by the peptide used for raising this antibody. TGF-beta (aa50) is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A 17-amino acid synthetic peptide from the carboxyl-terminus of rat TGF?.

Isotype: IgG1, kappa

Application Details

Application Notes: Immunohistology formalin-fixed 2-4 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1-2 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: Jurkat or Ramos cells. Testicular Carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGF-Alpha

Background:

Synonyms: EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1

Gene Symbol: TGFA

Molecular Weight: 6 kDa

Gene ID: 7039

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway