UGT1A9 antibody (CF®740)

Catalog No. ABIN7661612

The Mouse Monoclonal anti-UGT1A9 antibody has been validated for . It is suitable to detect UGT1A9 in samples from Human.

Quick Overview for UGT1A9 antibody (CF®740) (ABIN7661612)

Target: UGT1A9 (UDP Glucuronosyltransferase 1 Family, Polypeptide A9 (UGT1A9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UGT1A9 antibody is conjugated to CF®740

Application: Please inquire

Clone: UGT1A9-1229

Product Details anti-UGT1A9 Antibody (CF®740)

Purpose: UGT1A9 (UDP glucuronosyltransferase 1 family, polypeptide A9)(UGT1A9/1229), CF740 conjugate

Characteristics: This antibody recognizes a protein of about 60 kDa, which is identified as human UGT1A9. It does not cross-react with the other UGT1A isoforms including UGT1A7, UGT1A8, and UGT1A10 and shows a high degree of specificity. UGT1A9 is a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This MAb binds to human hepatocytes and the majority of human hepatocellular carcinomas (HCC s). In frozen sections, it stains hepatic cells and may be used as a marker of the liver. This MAb also binds to cell preparations of hepatocellular carcinoma biopsies or cell lines.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide specific to human UGT1A9 protein

Isotype: IgG2b, kappa

Application Details

Application Notes: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Comment:

Positive Control: HepG2 cells. Liver or Hepatocellular Carcinoma (HCC)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for UGT1A9

Target: UGT1A9 (UDP Glucuronosyltransferase 1 Family, Polypeptide A9 (UGT1A9))

Alternative Name: UGT1A9

Background:

Synonyms: GNT1, HLUGP4, lugP4, UDP-glucuronosyltransferase 1-I, UDP-glucuronosyltransferase 1A9 (UGT1A9), UDPGT 1-9, UDPGT, UGT-1I, UGT1*9, UGT1-09, UGT1.9, Ugt1a9, UGT1I

Gene Symbol: UGT1A9

Molecular Weight: 60 kDa

Gene ID: 54600

UniProt: O60656

Pathways: Steroid Hormone Biosynthesis, Regulation of Lipid Metabolism by PPARalpha