Uroplakin 3A antibody (AA 260-279) (CF®740)

Catalog No. ABIN7661693

The Rabbit Polyclonal anti-Uroplakin 3A antibody has been validated for WB. It is suitable to detect Uroplakin 3A in samples from Human.

Quick Overview for Uroplakin 3A antibody (AA 260-279) (CF®740) (ABIN7661693)

Target: Uroplakin 3A (UPK3A)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Uroplakin 3A antibody is conjugated to CF®740

Application: Western Blotting (WB)

Product Details anti-Uroplakin 3A Antibody (CF®740)

Binding Specificity: AA 260-279

Purpose: UPK3A (Uroplakin 3A)(Rabbit PAb), CF740 conjugate

Characteristics: Uroplakin-3A (UPK3A) is a component of the asymmetric unit membrane (AUM). It is a highly specialized bio-membrane made by terminally differentiated urothelial cells. The protein may play an important role in AUM-cytoskeleton interaction in terminally differentiated urothelial cells. UPK3A also contributes to the formation of urothelial glycocalyx, which may play an important role in preventing bacterial adherence through FimH bacterial protein binding leading to bladder infection. UPK3A has been shown as a helpful marker for the detection of bladder cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide, aa260-279 (GASESSYTSVNRGPPLDRAE) of human UPK3A

Isotype: IgG

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HEK293 cells or bladder carcinoma

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Uroplakin 3A

Target: Uroplakin 3A (UPK3A)

Alternative Name: UPK3A

Background:

Synonyms: UP3a, UPIIIA, UPK3A, Uroplakin III (UPIII), Uroplakin3A

Gene Symbol: UPK3A

Tissue Expression: Urothelial cells

Molecular Weight: ~30 kDa

Gene ID: 7380

UniProt: O75631