UACA antibody (CF®740)

Catalog No. ABIN7661694

The CF®740-conjugated Mouse Monoclonal anti-UACA antibody (Clone AE-5) (ABIN7661694) specifically detects UACA in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for UACA antibody (CF®740) (ABIN7661694)

Target: UACA (Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This UACA antibody is conjugated to CF®740

Application: Western Blotting (WB)

Clone: AE-5

Product Details anti-UACA Antibody (CF®740)

Purpose: UACA / Nucling(AE-5), CF740 conjugate

Characteristics: UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is a 1,416 amino acid nuclear membrane protein. It was originally identified as an autoantigen in patients with panuveitis, a characteristic of Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 embryonal carcinoma cells during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the cell-death pathway.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Nuclei of myeloid leukemia biopsy cells

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 0.5-1.0 μg/mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HeLa or 293T cells. Highly expressed in skeletal muscle, heart, kidney and pancreas. Also expressed in epidermal melanocytes, eye muscles and thyroid follicular cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for UACA

Target: UACA (Uveal Autoantigen With Coiled Coil Domains And Ankyrin Repeats (UACA))

Alternative Name: Nucling

Background:

Synonyms: Nuclear membrane binding protein, Uveal autoantigen with coiled-coil domains and ankyrin repeats (UACA), NUCLING

Gene Symbol: UACA

Molecular Weight: 160 kDa

Gene ID: 55075

UniProt: Q9BZF9

Pathways: Positive Regulation of Endopeptidase Activity