Villin 1 antibody (AA 179-311) (Biotin)

Catalog No. ABIN7661910

This anti-Villin 1 antibody is a Mouse Monoclonal antibody detecting Villin 1 in IHC and IHC (fp). Suitable for Human.

Quick Overview for Villin 1 antibody (AA 179-311) (Biotin) (ABIN7661910)

Target: Villin 1 (VIL1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Villin 1 antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: VIL1-2376

Product Details anti-Villin 1 Antibody (Biotin)

Binding Specificity: AA 179-311

Purpose: Villin (GI-Mucosal & Urogenital Brush Border Marker)(VIL1/2376), Biotin conjugate

Characteristics: Recognizes a protein of 95 kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A recombinant fragment (around aa 179-311) of human Villin protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: A549, HepG2 and HCT116 cells. Colon or Rectum.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for Villin 1

Target: Villin 1 (VIL1)

Alternative Name: Villin

Background:

Synonyms: VIL1, Villin-1, Villin1

Gene Symbol: VIL1

Tissue Expression: Epithelial cells|Intestine

Molecular Weight: 93 kDa

Gene ID: 7429

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization