Recombinant VWF antibody (AA 1815-1939) (CF®405S)

Catalog No. ABIN7661973

This anti-VWF antibody is a Mouse Monoclonal antibody detecting VWF in WB, IHC and IHC (fp). Suitable for Human.

Quick Overview for Recombinant VWF antibody (AA 1815-1939) (CF®405S) (ABIN7661973)

Target: VWF (Von Willebrand Factor (VWF))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VWF antibody is conjugated to CF®405S

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: RVWF-1465

Product Details anti-VWF Antibody (CF®405S)

Binding Specificity: AA 1815-1939

Purpose: von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) (rVWF/1465), CF405S conjugate

Characteristics: von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human vWF protein (aa1815-1939)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Positive Control: HUVEC cells. Tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for VWF

Target: VWF (Von Willebrand Factor (VWF))

Alternative Name: Von Willebrand Factor

Background:

Synonyms: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

Gene Symbol: VWF

Tissue Expression: Endothelial cells

Molecular Weight: 250 kDa

Gene ID: 7450

UniProt: P04275