VWF antibody (AA 845-949) (Biotin)

Catalog No. ABIN7662013

This Mouse Monoclonal antibody specifically detects VWF in IHC and IHC (fp). It exhibits reactivity toward Human.

Quick Overview for VWF antibody (AA 845-949) (Biotin) (ABIN7662013)

Target: VWF (Von Willebrand Factor (VWF))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VWF antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Clone: IIIE2-34

Product Details anti-VWF Antibody (Biotin)

Binding Specificity: AA 845-949

Purpose: von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker)(IIIE2.34), Biotin conjugate

Characteristics: von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Immunogen: Recombinant human vWF fragment spanning aa 845-949

Isotype: IgG1, kappa

Application Details

Application Notes: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistochemistry (formalin-fixed): 0.5-1.0 μg/mL for 30 minutes at RT. Immunoprecipitation: 0.5-1 μg/500 μg protein lysate. Western blot: 0.5-1.0 μg/mL. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Comment:

Positive Control: HUVEC cells. Tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Expiry Date: 24 months

Target Details for VWF

Target: VWF (Von Willebrand Factor (VWF))

Alternative Name: Von Willebrand Factor

Background:

Synonyms: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

Gene Symbol: VWF

Tissue Expression: Endothelial cells

Molecular Weight: 250 kDa

Gene ID: 7450

UniProt: P04275