CD3 epsilon antibody

Catalog No. ABIN7670007

This Golden Syrian Hamster Monoclonal antibody specifically detects CD3 epsilon in FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD3 epsilon antibody (ABIN7670007)

Target: CD3 epsilon (CD3E)

Reactivity: Mouse

Host: Golden Syrian Hamster

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: 500A2

Product Details anti-CD3 epsilon Antibody

Purpose: Purified Anti-Mouse CD3e Antibody[500A2]

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: ≥ 1 mg/mL

Buffer: Phosphate buffered solution, pH 7.2, containing 0.05 % proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze / thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.

Expiry Date: 12 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3epsilon

Background: T-cell surface glycoprotein CD3 epsilon chain,CD3E,T-cell surface antigen T3/Leu-4 epsilon chain,CD3e,CD3E,T3E,Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development. Initiates the TCR-CD3 complex assembly by forming the two heterodimers CD3D/CD3E and CD3G/CD3E. Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region

UniProt: P22646

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway