IL-2 antibody

Catalog No. ABIN7670184

This Rat Monoclonal antibody specifically detects IL-2 in FACS. It exhibits reactivity toward Human.

Quick Overview for IL-2 antibody (ABIN7670184)

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: MQ1-17H12

Product Details anti-IL-2 Antibody

Purpose: AF/LE Purified Anti-Human IL-2 Antibody[MQ1-17H12]

Purification: >98%, Protein A/G purified

Endotoxin Level: Low Endotoxin

Immunogen: Recombinant Human IL-2 protein

Isotype: IgG2a, kappa

Application Details

Application Notes: FCM 2 μg/mL(1x10⁵-5x10⁵ cells)

Restrictions: For Research Use only

Handling

Concentration: ≥ 1 mg/mL

Buffer: Sterile PBS, pH 7.2. < 1.0 EU per mg of the antibody as determined by the LAL method.

Preservative: Azide free

Handling Advice: avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Expiry Date: 12 months

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2

Background: IL2,IL-2,TCGF,lymphokine,Aldesleukin,IL-2lymphokine,interleukin-2,Proleukin,interleukin 2,involved in regulation of T-cell clonal expansion,T cell growth factor,T-cell growth factor,IL 2,IL2,Interleukin 2,interleukin2,Involved in regulation of T cell clonal expansion,Lymphokine,POIL2,T Cell Growth Factor,TCGF,Interleukin-2 (IL-2) is a O-glycosylated, four alpha -helix bundle cytokine that has potent stimulatory activity for antigen-activated T cells. It is expressed by CD4+ and CD8+ T cells, gamma δ T cells, B cells, dendritic cells, and eosinophils. Mature human IL-2 shares 56 % and 66 % aa sequence identity with Mouse and Rat IL-2, respectively. Human and Mouse IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. The 55 kDa IL-2 R alpha is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 R beta, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain gamma c/IL-2 R gamma, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 R beta and gamma c. IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells to prolifeRate and induces IL-2 and IL-2 R alpha synthesis. It contributes to T cell homeostasis by promoting the Fas-induced death of naive CD4+ T cells but not activated CD4+ memory lymphocytes. IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity.

UniProt: P60568

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation