MCAM antibody (PE)

Catalog No. ABIN7670248

The Rat Monoclonal anti-MCAM antibody has been validated for FACS. It is suitable to detect MCAM in samples from Mouse.

Quick Overview for MCAM antibody (PE) (ABIN7670248)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This MCAM antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: ME-9F1

Product Details anti-MCAM Antibody (PE)

Purpose: PE Anti-Mouse CD146 Antibody[ME-9F1]

Immunogen: E-AB-F09832D

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 5 μL/tests

Buffer: Phosphate buffered solution, pH 7.2, containing 0.09 % stabilizer and 1 % protein protectant

Handling Advice: Please protected from prolonged exposure to light and do not freeze.

Storage: 4 °C

Storage Comment: This product can be stored at 2-8°C for 12 months.

Expiry Date: 12 months

Target Details for MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Alternative Name: CD146

Background: S-Endo 1 antigen,MUC18,MCAM,Mel-CAM,A32 antigen,CD146, also known as melanoma cell adhesion molecule (MCAM or Mel-CAM), MUC18, S-Endo1, and A32 antigen, is an integral membrane glycoprotein that belongs to the Ig superfamily. CD146 is strongly expressed by murine vascular endothelial cells. It is expressed on about 30 % of neutrophils and 60 % of NK cells. Unlike in humans, CD146 is undetectable on monocytes, dendritic cells, T cells, NKT cells, B cells, or smooth muscle cells in mouse. It has been reported that an increase in CD146 expression is associated with NK cell maturation. Combined with using CD27 and CD11b staining, CD146 may be an alternative marker to detect final stages of NK cell maturation and define NK cell subsets. CD146+ NK cells were found to be less cytotoxic and to produce less IFNγ than CD146- NK cells upon stimulation with target cells or activating antibodies. The role of CD146 on NK cell migration has yet to be investigated. The identification of CD146 ligand(s) will be crucial to address this issue.

UniProt: P10810