SIRPA antibody (FITC)

Catalog No. ABIN7673078

This Mouse Monoclonal antibody specifically detects SIRPA in FACS. It exhibits reactivity toward Human.

Quick Overview for SIRPA antibody (FITC) (ABIN7673078)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPA antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: SE5A5

Product Details anti-SIRPA Antibody (FITC)

Purpose: FITC Anti-Human CD172a/b Antibody

Purification: 0

Purity: 0

Isotype: IgG1, kappa

Application Details

Application Notes: Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0. 1 % sodium azide, 1 % BSA, pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: 4 °C

Storage Comment: 2-8°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172a

Background:

Synonyms: SIRPa|SIRPb|SIRPalpha/beta|BIT|SHPS1|MFR|P84|PTPNS1|CD172 antigen-like family member A|CD172 antigen-like family member B

Background: CD172a, also known as signal-regulatory protein α (SIRPα), src homology 2 domain-containing phosphatase substrate-1 (SHPS1), PTPNS1, BIT, MFR, and P84, is a 75-110 kD transmembrane glycoprotein involved in receptor tyrosine kinase coupled signaling pathway. It belongs to the Ig superfamily and is primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, and neurons. CD172a serves as a substrate of activated receptor tyrosine kinases (RTKs). The interaction of CD172a intracellular domain with SHP-1 and SHP-2 displays negative signaling in the regulation of leukocyte adhesion and transmigration, T cell activation, macrophage fusion, and phagocytosis. CD47 (IAP) is the extracellular ligand for CD172a. SIRPα was recently demonstrated to be a specifc marker for cardiomyocytes derived from human pluripotent stem cells2.

Molecular Weight: 0

Gene ID: 140885

UniProt: O00241