EpCAM antibody

Catalog No. ABIN7674792

This Mouse Monoclonal antibody specifically detects EpCAM in FACS, D and Act. It exhibits reactivity toward Human.

Quick Overview for EpCAM antibody (ABIN7674792)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EpCAM antibody is un-conjugated

Application: Flow Cytometry (FACS), Detection (D), Activation (Act)

Clone: MOC-181

Product Details anti-EpCAM Antibody

Purpose: The antibody MOC-181 is directed against human EpCAM. The antibody has been reported to react with an epitope within the first EGF-like repeat in the extracellular region of EpCAM.

Specificity: The antibody MOC-181 is directed against human EpCAM. The antibody has been reported to react with an epitope within the first EGF-like repeat in the extracellular region of EpCAM.

Characteristics: The epithelial cell adhesion molecule (EpCAM), also known as CD326, is a 40 kD glycoprotein that is expressed at the basolateral cell membrane of simple, pseudo-stratified and transitional epithelia of normal adult tissues. EpCAM is reported to function as a homotypic calcium-independent cell adhesion molecule but studies suggest that EpCAM is also involved in cellular processes such as cell signalling, migration, proliferation and differentiation. EpCAM is overexpressed in a wide range of human epithelial carcinomas including lung, colorectal, breast, prostate, head and neck, and hepatic carcinomas. The EpCAM molecule has, therefore, been widely used as a diagnostic marker for human carcinomas and has been considered as a potential target for immunotherapy. Since EpCAM is also present on exosomes, microvesicles abundantly secreted by tumor cells, antibodies to this cell surface antigen are widely used for the isolation and identification of these cancer derived exosomes. Apart from EpCAM 's role in cancer syndromes, homozygous mutations in the EpCAM gene have been identified in patients suffering from congenital tufting enteropathy, a rare autosomal recessive form of intractable diarrhea of infancy. Finally, EpCAM expression may be causally related to maintaining stem cells, and therefore will be a valuable target for future avenues to generate human induced pluripotent stem cells.

Purification: Purified

Immunogen: MOC-181 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with an extract from a small cell lung carcinoma cell line.

Isotype: IgG2a

Application Details

Application Notes: The MOC-181 antibody is suitable for the detection of human EpCAM by flow cytometry. Furthermore, MOC-181 has been shown to effectively induce tumor-specific myeloid cell activation in a mouse model. Optimal antibody dilutions for the different applications should be determined by titration.

Restrictions: For Research Use only

Handling

Reconstitution: Each vial contains 100 μg 1 mg/mL purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09 % sodium azide.

Buffer: Each vial contains 100 μg 1 mg/mL purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Target Details for EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Alternative Name: EpCAM