Cytokeratin 5 antibody (AA 331-430) (Biotin)

Catalog No. ABIN737063

The Rabbit Polyclonal anti-Cytokeratin 5 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect Cytokeratin 5 in samples from Human, Mouse and Rat.

Quick Overview for Cytokeratin 5 antibody (AA 331-430) (Biotin) (ABIN737063)

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 5 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cytokeratin 5 Antibody (Biotin)

Binding Specificity: AA 331-430

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CK5

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C for 12 months.

Expiry Date: 12 months

Target Details for Cytokeratin 5

Target: Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

Alternative Name: Cytokeratin 5

Background:

Synonyms: Cytokeratin 5, Keratin, type II cytoskeletal 5, CK-5, CK 5, CK5, Keratin-5, K5, 58 kDa cytokeratin, KRT5, DDD, EBS 2, EBS2, K5, Keratin 5 epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types, Keratin 5, Keratin Type II Cytoskeletal 5, Keratin5, KRT 5, KRT 5A, KRT5, Cytokeratin-5, Cytokeratin5, DDD, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, K2C5_HUMAN, Keratin 5, Keratin, Keratin Type II Cytoskeletal 5, Keratin5, KRT5A, type II cytoskeletal 5, Type-II keratin Kb5.

Background: Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinising tissue (ie., skin) and non keratinising cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. At least 20 different cytokeratins (CK) in the molecular range of 40 to 70 kDa and isoelectric points of 5 to 8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Defects in KRT5 are a cause of epidermolysis bullosa simplex.

Molecular Weight: 64kDa

Gene ID: 3852