Influenza Virus Nucleoprotein antibody (Influenza A Virus)

Catalog No. ABIN7795017

The Mouse Monoclonal anti-Influenza Virus Nucleoprotein antibody is suitable to detect Influenza Virus Nucleoprotein in samples from Influenza A Virus. It has been validated for ELISA, IF and IHC.

Quick Overview for Influenza Virus Nucleoprotein antibody (Influenza A Virus) (ABIN7795017)

Target: Influenza Virus Nucleoprotein (IV NP)

Reactivity: Influenza A Virus

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: IA-3808

Product Details

Purpose: Anti-Influenza A, Nucleocapsid

Specificity: Anti-Influenza A, Nucleoprotein (Clone IA-3808) is reactive with >50 separate isolates from H1 through H14. Background There are four types of Influenza (flu) viruses type A, B, C, and D. Influenza A and B viruses are routinely spread in people and other mammals, while also known for seasonal flu epidemics each year. Influenza A is the genus Alphainfluenzavirus of the virus family Orthomyxoviridae. Influenza A is classified into two main protein subtypes, hemagglutinin (HA) and neuraminidase (NA), located on the surface of the virus. Breaking down the proteins further, there are 18 hemagglutinin subtypes and 11 different neuraminidase subtypes1. Influenza A can be even further classified into specific clades (also known as groups) and sub-clades (also known as sub-groups) based on similarity of their HA/NA gene sequences1. The Nucleocapsid protein or nucleoprotein (NP) of the influenza virus A negative-strand RNA?s primary function is to encapsulate the virus genome for the purpose of RNA transcription, replication and packaging2. Specifically, NP is the most abundant viral protein in infected cells2, therefore the NP can be and has been used for anti-influenza drug development3. The NP of influenza A and B viruses share up to 38 % of their amino acid sequence, indicating region functionality differences at the amino acid level4.

Purification: This monoclonal antibody is purified from ascites fluid or culture medium by protein A chromatography or sequential differential precipitations.

Purity: >90 % monomer by analytical SEC and SDS-Page

Isotype: IgG2a

Application Details

Application Notes: ELISA Recommended starting range 1:20-1:200 IF Recommended starting range 1:10-1:50 IHC

Restrictions: For Research Use only

Handling

Concentration: 1 μg/mL

Buffer: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1 % sodium azide. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: This product contains no stabilizing proteins and should be stored at 2-8°C until ready to use.

Target Details

Target: Influenza Virus Nucleoprotein (IV NP)

Alternative Name: Influenza A Nucleocapsid

Target Type: Influenza Protein

Background: Common flu NP, Influenza A virus NP, NP, Nucleocapsid protein, Nucleoprotein, Protein N, Seasonal Influenza A (H1N1) Nucleocapsid Protein,