Recombinant PD-1 antibody

Catalog No. ABIN7795244

This Mouse Monoclonal antibody specifically detects PD-1 in FACS, WB, IHC, ELISA, ICC and in vivo. It exhibits reactivity toward Mouse.

Quick Overview for Recombinant PD-1 antibody (ABIN7795244)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), In vivo Studies (in vivo)

Grade: In vivo Grade

Clone: 29F-1A12-1

Product Details anti-PD-1 Antibody

Expression System: Mammalian cells

Purpose: In Vivo Grade Recombinant Anti-mouse PD-1 Monoclonal Antibody (Clone 29F.1A12.1), Mouse IgG2a-L234A L235A P329G (LALAPG) Kappa

Characteristics: The rat anti-mouse PD-1 monoclonal antibody 29F.1A12 (rat IgG2a kappa) reacts with the mouse PD-1 protein (programmed death-1 or CD279) encoded by the mouse Pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 29F.1A12 monoclonal antibody blocks the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein. Our recombinant 29F.1A12 antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD-1 monoclonal antibody (hybridoma clone name or number: 29F.1A12).

Purification: Protein A or G affinity column

Purity: >95 % by reducing SDS-PAGE

Endotoxin Level: < 1 EU per 1 mg of the protein by the LAL method

Immunogen: The original rat hybridoma (clone name: 29F.1A12) was generated by immunizing rats with the mouse PD-1 cDNA followed by the mouse PD-1-Ig fusion protein.

Isotype: IgG2a, L234A, L235A, P329G

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: > 3 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PD-1

Pathways: Cancer Immune Checkpoints