PD-1 antibody

Catalog No. ABIN5526891

The Mouse Monoclonal anti-PD-1 antibody is suitable to detect PD-1 in samples from Human and Non-Human Primate. It has been validated for FACS and IHC (fro).

Quick Overview for PD-1 antibody (ABIN5526891)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This PD-1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: EH12-2H7

Product Details anti-PD-1 Antibody

Purpose: Anti-Hu CD279 Purified

Cross-Reactivity (Details): Human, Non-Human Primates

Characteristics: The mouse monoclonal antibody EH12.2H7 recognizes an extracellular epitope of CD279 / PD-1 (programmed cell death 1), a 55 kDa type I transmembrane protein expressed above all during T cell development, on activated T cells, activated B cells, and activated monocytes.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: recombinant human CD279

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: CD279

Background: Programmed cell death 1,CD279 / PD-1 (programmed cell death 1), a transmembrane protein of CD28/CTLA-4 family. It is expressed inducibly mainly on activated T, B, and myeloid cells and plays a role in maintaining peripheral self-tolerance. Binding to its ligands CD273 and CD274 is associated with inhibition of T cell proliferation and induction of their anergy. It is also expressed during thymic development. Some variants of CD279 are associated with susceptibility to systemic lupus erythematosus, type 1 diabetes, and rheumatoid arthritis.,PDCD1, PD1, SLEB2

Gene ID: 5133

UniProt: Q15116

Pathways: Cancer Immune Checkpoints