Recombinant PD-L1 antibody

Catalog No. ABIN7795533

The Mouse Bispecific anti-PD-L1 antibody is suitable to detect PD-L1 in samples from Mouse. It has been validated for WB, FACS, IHC, ELISA, ICC and in vivo.

Quick Overview for Recombinant PD-L1 antibody (ABIN7795533)

Target: PD-L1 (CD274 (PD-L1))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Mouse

Clonality: Bispecific

Conjugate: This PD-L1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), In vivo Studies (in vivo)

Grade: In vivo Grade

Clone: 10F-9G2-1-29F-1A12-1

Product Details anti-PD-L1 Antibody

Expression System: Mammalian cells

Purpose: In Vivo Grade Recombinant Anti-mouse PD-L1 / PD-1 Bispecific Antibody, Mouse IgG2c LALAPG Kappa (Clone 10F.9G2.1 / 29F.1A12.1)

Characteristics: The rat anti-mouse PD-1 monoclonal antibody 29F.1A12 (rat IgG2a kappa) reacts with the mouse PD-1 protein (programmed death-1 or CD279) encoded by the mouse Pdcd1 gene, a member of the CD28 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2, both of which belong to the B7 family. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 29F.1A12 monoclonal antibody blocks the binding of both the mouse PD-L1 protein and the mouse PD-L2 protein to the mouse PD-1 protein. The rat anti-mouse PD-L1 monoclonal antibody 10F.9G2 (rat IgG2b kappa) reacts with the mouse PD-L1 protein (programmed death ligand-1, B7-H1 or CD274), a member of the B7 family of the Ig superfamily. PD-1 has two ligands, PD-L1 and PD-L2. It has been shown that in mouse models of melanoma, tumor growth can be transiently arrested via treatment with the anti-mouse PD-1 and anti-mouse PD-L1 antibodies which block the interaction between the PD-L1 protein and its receptor PD-1 protein. The 10F.9G2 monoclonal antibody blocks the binding of the mouse PD-L1 protein to the mouse PD-1 protein. It is under investigation how the recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies (29F.1A12 / 10F.9G2 and 10F.9G2 / 29F.1A12) behave different from the individual monoclonal antibodies and their combination (29F.1A12 + 10F.9G2). Our recombinant anti-mouse PD-1 / PD-L1 bispecific antibodies have a part (variable regions) or complete amino acid sequences of the rat anti-mouse PD-1 antibody (hybridoma clone name or number: 29F.1A12) and the rat anti-mouse PD-L1 antibody (hybridoma clone name or number: 10F.9G2).

Purification: Protein A or G affinity column

Purity: >95 % by reducing SDS-PAGE

Endotoxin Level: < 1 EU per 1 mg of the protein by the LAL method

Isotype: IgG2c, L234A, L235A, P329G, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: > 3 mg/mL

Storage: 4 °C,-20 °C

Storage Comment: Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PD-L1 & PD-1

Sub Type: Bispecific

Pathways: Cancer Immune Checkpoints