Recombinant ARID1A antibody (AA 750-800)

Catalog No. ABIN7795677

The Rabbit Monoclonal anti-ARID1A antibody is suitable to detect ARID1A in samples from Human and Mouse. It has been validated for WB, IHC, ICC, FACS and IP.

Quick Overview for Recombinant ARID1A antibody (AA 750-800) (ABIN7795677)

Target: ARID1A (AT Rich Interactive Domain 1A (SWI-Like) (ARID1A))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This ARID1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: BLR279L

Product Details anti-ARID1A Antibody

Binding Specificity: AA 750-800

Purpose: Rabbit anti-ARID1A/BAF250 Recombinant Monoclonal Antibody [BLR279L]

Purification: Purified

Immunogen: Between 750 and 800

Isotype: IgG

Application Details

Application Notes: Flow-Cyt,Fixed in 4 % formaldehyde and permeabilized with 90 % methanol. 1 μL per 1 x 10^6 cells.,ICC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.,IHC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.,IP,20 μL/mg lysate ,WB,11000 (3-8 % Tris-acetate gel recommended)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 50 μg/mL

Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details for ARID1A

Target: ARID1A (AT Rich Interactive Domain 1A (SWI-Like) (ARID1A))

Alternative Name: ARID1A/BAF250

Background: The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. SWI/SNF complexes possess ATPase and DNA helicase activity that promotes transcriptional activation of genes normally repressed by chromatin structure. ARID1A/BAF250 is a noncatalytic subunit that is a member of the ARID (AT-rich interactive domain-containing protein) family of DNA binding proteins. ARID1A/BAF250 has been shown to play a role in the tumor suppressor activity of the SWI/SNF complexes. ARID1A/BAF250 is required for differentiation-associated cell cycle arrest.

Gene ID: 8289

UniProt: O14497

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Tube Formation