Recombinant NF-KappaB2 (/p52) (AA 1-50) antibody

Catalog No. ABIN7795782

The Rabbit Monoclonal anti-NF-KappaB2 (/p52) antibody is suitable to detect NF-KappaB2 (/p52) in samples from Human. It has been validated for FACS, ICC, IHC, IP and WB.

Quick Overview for Recombinant NF-KappaB2 (/p52) (AA 1-50) antibody (ABIN7795782)

Target: NF-KappaB2 (/p52)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)

Clone: BLR350N

Product Details

Binding Specificity: AA 1-50

Purpose: Rabbit anti-NF-kappaB2 (p100/p52) Recombinant Monoclonal Antibody [BLR350N]

Purification: Purified

Immunogen: between 1 and 50

Isotype: IgG

Application Details

Application Notes: WB,11000,ICC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.,IHC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.,IP,6 μL/mg lysate,Flow-Cyt,Fixed in 4 % formaldehyde and permeabilized with 90 % methanol. 1 μL per 1 x 10^6 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1000 μg/mL

Buffer: Phosphate Buffered Saline (PBS) with 0.09 % Sodium Azide, BSA-Free

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8 °C

Expiry Date: 12 months

Target Details

Target: NF-KappaB2 (/p52)

Background: The NFKB2 gene encodes a 100 kDa protein that can undergo co-translational processing to also yield a 52 kDa protein. The 52 kDa protein is also known as NF-kappa B2/p52 and is one of five monomeric subunits that are part of the Rel family of proteins that dimerize to form the NF-kappa B (nuclear factor that binds the kappa immunoglobulin light chain gene enhancer) transcription factor. Members of the Rel family of proteins include NF-kappa B1/p50, NF-kappa B2/p52, RelA/p65, c-Rel, and RelB. The 100 kDa product is also known as p100 or I kappa B delta. P100/I kappa B delta has been shown to function as an inhibitor protein that interacts with NF-kappa B and inhibit its DNA binding activity. P100/I kappa B delta is a member of the I kappa B family of proteins which includes I kappa B-beta, I kappa B-delta/NF-kappa B2/p100, I kappa B-gamma/NF-kappa B1/p105, I kappa B-epsilon, I kappa B-zeta, and Bcl3. The classical description of the NF-kappa B transcription factor is a transcription factor that reprograms gene expression of the immune system in response to injury, infection, and stress.

Gene ID: 4791

UniProt: Q00653