Recombinant BUB3 antibody

Catalog No. ABIN7797806

This Rabbit Monoclonal antibody specifically detects BUB3 in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant BUB3 antibody (ABIN7797806)

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This BUB3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 25GB3085

Product Details anti-BUB3 Antibody

Expression System: HEK-293 Cells

Purpose: KD-Validated BUB3 Mitotic Checkpoint Protein Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Bub3

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for BUB3

Target: BUB3 (Budding Uninhibited By Benzimidazoles 3 Homolog (Yeast) (BUB3))

Alternative Name: BUB3 Mitotic Checkpoint Protein

Molecular Weight: 37 kDa

Gene ID: 9184

UniProt: O43684

Pathways: Maintenance of Protein Location