Recombinant eIF4EBP1/2/3 (pThr32), (pThr46) antibody

Catalog No. ABIN7798429

The Rabbit Monoclonal anti-eIF4EBP1/2/3 antibody (Clone 23GB3850) (ABIN7798429) specifically detects eIF4EBP1/2/3 in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for Recombinant eIF4EBP1/2/3 (pThr32), (pThr46) antibody (ABIN7798429)

Target: eIF4EBP1/2/3

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 23GB3850

Product Details

Binding Specificity: pThr32, pThr46

Expression System: HEK-293 Cells

Purpose: KD-Validated Phospho-eIF4EBP1/2/3 (T46+T46+T32) Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Phospho-eIF4EBP1/2/3 (T46+T46+T32)

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS (pH 7.4) containing 50% glycerol and 0.02% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details

Target: eIF4EBP1/2/3

Molecular Weight: 13 kDa

Gene ID: 1978, 1979, 8637

UniProt: Q13541, Q13542, O60516