EGFR antibody

Catalog No. ABIN7798473

The Mouse Monoclonal anti-EGFR antibody has been validated for WB and FACS. It is suitable to detect EGFR in samples from Human.

Quick Overview for EGFR antibody (ABIN7798473)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This EGFR antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 24GB5790

Product Details anti-EGFR Antibody

Purpose: KD-Validated Epidermal Growth Factor Receptor Mouse mAb

Purification: Affinity Purified

Immunogen: Recombinant protein of human EGFR

Isotype: IgG1

Application Details

Application Notes: Western Blotting (WB):1:2,500-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for EGFR

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: Epidermal Growth Factor Receptor

Molecular Weight: 134 kDa

Gene ID: 1956

UniProt: P00533

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins