Recombinant Cytokeratin 18 antibody

Catalog No. ABIN7799174

This Rabbit Monoclonal antibody specifically detects Cytokeratin 18 in WB, FACS and IF (p). It exhibits reactivity toward Human.

Quick Overview for Recombinant Cytokeratin 18 antibody (ABIN7799174)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Grade: KD Validated

Clone: 25GB2535

Product Details anti-Cytokeratin 18 Antibody

Expression System: HEK-293 Cells

Purpose: KD-Validated Keratin 18 Recombinant Rabbit mAb

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Cytokeratin 18

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: Keratin 18

Molecular Weight: 48 kDa

Gene ID: 3875

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis