Recombinant Notch1 antibody

Catalog No. ABIN7799625

The Rabbit Monoclonal anti-Notch1 antibody has been validated for WB and FACS. It is suitable to detect Notch1 in samples from Human.

Quick Overview for Recombinant Notch1 antibody (ABIN7799625)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 25GB425

Product Details anti-Notch1 Antibody

Expression System: HEK-293 Cells

Purpose: KD-Validated NOTCH1 Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Notch 1

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: NOTCH1

Molecular Weight: 125 kDa

Gene ID: 4851

UniProt: P46531

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development