PHGDH antibody

Catalog No. ABIN7799808

The Mouse Monoclonal anti-PHGDH antibody has been validated for WB, FACS and IF (p). It is suitable to detect PHGDH in samples from Human.

Quick Overview for PHGDH antibody (ABIN7799808)

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PHGDH antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Grade: KD Validated

Clone: 24GB14070

Product Details anti-PHGDH Antibody

Purpose: KD-Validated PHGDH Mouse mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: Recombinant protein of human PHGDH

Isotype: IgG2a

Application Details

Application Notes: Western Blotting (WB): 1:500-1:2,500, Flow Cytometry (FCM): 1:200-1:2,000, Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: PHGDH

Molecular Weight: 57 kDa

Gene ID: 26227

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect