Recombinant PKC gamma antibody (pThr514)

Catalog No. ABIN7799998

The Rabbit Monoclonal anti-PKC gamma antibody (Clone 24GB4490) (ABIN7799998) specifically detects PKC gamma in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for Recombinant PKC gamma antibody (pThr514) (ABIN7799998)

Target: PKC gamma (PRKCG) (Protein Kinase C, gamma (PRKCG))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PKC gamma antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 24GB4490

Product Details anti-PKC gamma Antibody

Binding Specificity: pThr514

Expression System: HEK-293 Cells

Purpose: KD-Validated Phospho-PKC gamma (Thr514) Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Phospho-PKC gamma (Thr514)

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for PKC gamma

Target: PKC gamma (PRKCG) (Protein Kinase C, gamma (PRKCG))

Alternative Name: PKC gamma

Molecular Weight: 78 kDa

Gene ID: 5582

UniProt: P05129

Pathways: WNT Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, G-protein mediated Events, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGF Signaling