Recombinant Src antibody (pTyr419)

Catalog No. ABIN7800050

This Rabbit Monoclonal antibody specifically detects Src in WB and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant Src antibody (pTyr419) (ABIN7800050)

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Src antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 23GB4080

Product Details anti-Src Antibody

Binding Specificity: pTyr419

Expression System: HEK-293 Cells

Purpose: KD-Validated Phospho-Src (Y419) Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Phospho-Src (Y419)

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for Src

Target: Src (Proto-oncogene tyrosine-protein kinase Src (Src))

Alternative Name: Src

Molecular Weight: 60 kDa

Gene ID: 6714

UniProt: P12931

Pathways: JAK-STAT Signaling, Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling, Platelet-derived growth Factor Receptor Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling