Recombinant SMAD2 antibody (pSer250)

Catalog No. ABIN7800370

The Rabbit Monoclonal anti-SMAD2 antibody (Clone 23GB4150) (ABIN7800370) specifically detects SMAD2 in WB and FACS. The antibody is reactive with Human samples.

Quick Overview for Recombinant SMAD2 antibody (pSer250) (ABIN7800370)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Grade: KD Validated

Clone: 23GB4150

Product Details anti-SMAD2 Antibody

Binding Specificity: pSer250

Expression System: HEK-293 Cells

Purpose: KD-Validated Phospho-Smad2 (S250) Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Phospho-Smad2 (S250)

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: Smad2

Molecular Weight: 52 kDa

Gene ID: 4087

UniProt: Q15796

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus