Recombinant Topoisomerase II alpha antibody

Catalog No. ABIN7800574

This anti-Topoisomerase II alpha antibody is a Rabbit Monoclonal antibody detecting Topoisomerase II alpha in WB, FACS and IF (p). Suitable for Human.

Quick Overview for Recombinant Topoisomerase II alpha antibody (ABIN7800574)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Grade: KD Validated

Clone: 24GB1915

Product Details anti-Topoisomerase II alpha Antibody

Expression System: HEK-293 Cells

Purpose: KD-Validated Topoisomerase II alpha Recombinant Rabbit mAb

Cross-Reactivity: Mouse, Rat

Purification: Affinity Purified

Immunogen: A synthesized peptide derived from human Topoisomerase II alpha

Isotype: IgG

Application Details

Application Notes: Western Blotting (WB): 1:1,000-1:5,000, Flow Cytometry (FCM): 1:200-1:2,000, Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200

Comment:

We assess the antibodies’ specificity and selectivity using knockdown techniques. Our validation approach is straightforward: if an antibody identifies a signal in wild-type (WT) cells, but this signal is absent or noticeably reduced in gene-silenced (KD) cells, we consider the antibody to be highly specific.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C for one year.

Expiry Date: 12 months

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: Topoisomerase II alpha

Molecular Weight: 174 kDa

Gene ID: 7153

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases