Cyclin D2 antibody (AA 161-288)

Catalog No. ABIN738366

This anti-Cyclin D2 antibody is a Rabbit Polyclonal antibody detecting Cyclin D2 in WB, ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). Suitable for Human, Mouse and Rat. This Primary Antibody has been cited in 1 publication.

Quick Overview for Cyclin D2 antibody (AA 161-288) (ABIN738366)

Target: Cyclin D2 (CCND2)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Cyclin D2 Antibody

Binding Specificity: AA 161-288

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Cyclin D2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Cyclin D2 antibody (ABIN738366)

Wang, Wu, Gao, Chen, Gu, Ding, Guo: "Elevated toll-like receptor 3 inhibits pancreatic ?-cell proliferation through G1 phase cell cycle arrest." in: Molecular and cellular endocrinology, Vol. 377, Issue 1-2, pp. 112-22, (2013) (PubMed).

Target Details for Cyclin D2

Target: Cyclin D2 (CCND2)

Alternative Name: Cyclin D2

Background:

Synonyms: MPPH3, KIAK2, G1/S-specific cyclin-D2, CCND2

Background: Regulatory component of the cyclin D2-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D2/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).

Gene ID: 894

UniProt: P30279

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases